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Temperature (C): Some discharge curves include data at different operating temperatures to assess thermal performance

Base editing-mediated gene knockout in the embryo To trace PGC fate after Trim37 knockout, we adopted a base editor previously reported in our recent work 33 to efficiently inactivate genes in zygotes

Peroxynitrite (ONOO - ,0.1 M stock solution in 0.3 M NaOH-NaCl) was diluted to less than 1/100 with ice-cold water, just prior to quick exposure to HGF in PBS by vortexing at 1:2000 (see Figure 1a for the experimental design) 32

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