The amount varies depending on age, dietary intake, and medical conditions
Simultaneously, GHK-Cu stimulates the production of decorin, a proteoglycan that regulates collagen fibril assembly and organization, ensuring that newly synthesized collagen forms properly organized matrices rather than disorganized scar tissue

In a 1992 study in rats, the major finding was that intraperitoneal DSIP injection increased the concentration of substance P a neurotransmitter positively correlated with the regulation of anxiety and mood15 in the hypothalamus, suggesting to the researchers that the peptide sharply decreases the classical manifestations of stress.16 Earlier studies in humans, namely a series of five trials published in 1983, came to similar conclusions, with better relaxation and apparently improved tolerance against psychic stress reported by the subjects.17 Its possible that the improved stress and anxiety markers were consequent to DSIP-associated sleep enhancements.18 Substance use recovery A study from 1984 published in European Neurology describes DSIPs potential use as a treatment for alcohol and opiate use disorders
As a result, a single vial of bacteriostatic water can be used for multiple dose preparations over time (up to 28 days after opening) without significant risk of contamination